下肢缺血是研究血管再生和缺血性血管新生的通用實(shí)驗模型，廣泛應用于外周血管疾病、重度下肢缺血、糖尿病足、截肢水平、血管新生、缺血保護與耐受、缺血再灌注損傷等方面的研究與評價(jià)，同時(shí)也是下肢缺血動(dòng)物模型制備中必要的評價(jià)手段。
激光多普勒成像可被用于無(wú)創(chuàng )評價(jià)大小鼠下肢的系統性血流灌注，同時(shí)也不需要追蹤染料。這使我們可以將結扎限流的下肢血流與對照下肢進(jìn)行比較。檢測可在很多天內持續進(jìn)行，因此可以評價(jià)由血管再生而產(chǎn)生的再灌注進(jìn)程。對于促血管再生性治療，血流將快速恢復到基線(xiàn)（100%），即與對照肢相同的水平。對于抑制血管再生性實(shí)驗，血流將更慢地恢復到正常的基線(xiàn)水平。
缺血手術(shù)后血流恢復狀況
相關(guān)產(chǎn)品
moorLDI2-HIR點(diǎn)掃描激光多普勒成像系統 moorFLPI-2激光散斑血流成像
References
Aicher A., Heeschen C., Sasaki K., Urbich C., Zeiher A. M., Dimmeler S. 2006
Low-energy shock wave for enhancing recruitment of endothelial progenitor cells: a new modality to increase efficacy of cell therapy in chronic hind limb ischemia.
Circulation, 114 (25), pp 2823-30
Chalothorn D, Zhang H, Clayton J. A, Thomas S. A, Faber J. E, 2005.
Catecholamines augment collateral vessel growth and angiogenesis in hindlimb ischemia.
American Journal of Physiology - Heart and Circulatory Physiology Published 1 August 2005 Vol. 289 no. 2, H947-H959.
Crawford R. S., Albadawi H., Atkins M. D., Jones J. E., Yoo H. J., Conrad M. F., Austen Jr W. G., Watkins M. T. 2010
Postischemic poly (ADP-ribose) polymerase (PARP) inhibition reduces ischemia reperfusion injury in a hind-limb ischemia model.
Surgery, 148 (1), pp 110 - 118
Tritsaris K., Myren M., Ditlev S. B., Hübschmann M. V., van der Blom I., Hansen A. J., Olsen U. B., Cao R. , Zhang J., Jia T., Wahlberg E., Dissing S., Cao Y. 2007
L-20 is an arteriogenic cytokine that remodels collateral networks and improves functions of ischemic hind limbs.
Proc Natl Acad Sci U S A.104 (39), pp 15364-9
Turgeon J., Dussault S., Haddad P., Groleau J., Ménard C, Michaud S. E., Maingrette F., Rivard A. 2010
Probucol and antioxidant vitamins rescue ischemia-induced neovascularization ………. exposed to cigarette smoke: Potential role of endothelial progenitor cells.
Atherosclerosis 208, spp 342–349